Free tool

NAD+ reconstitution calculator

Pre-filled for a 500 mg NAD+ vial with 5 mL of BAC water: 100,000 mcg/mL, or 1 mg per unit on a U-100 syringe. Change the vial or water to match yours, and add an amount to see the units to draw.

Updated October 2026

Calculator

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Opens PeptidesAI with this vial and water filled in. You add your own amount.

This is a math tool. It converts amounts; it does not tell you what amount to use. NAD+ vials sold as research chemicals are not FDA-approved products, and nothing here is medical advice.

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How the NAD+ math works

A 500 mg vial holds 500,000 mcg of NAD+. Adding 5 mL of bacteriostatic water spreads that over 5 mL, so the solution is 500,000 ÷ 5 = 100,000 mcg per mL. A U-100 insulin syringe has 100 units per mL, so each unit is 0.01 mL and holds 100,000 × 0.01 = 1 mg.

To turn an amount into units, divide it by the amount per unit. The calculator above does this for you, and warns you when a draw is too small to measure or bigger than the syringe.

NAD+ concentration by vial size and water volume

Each cell shows the concentration and how much one U-100 unit holds. The app's reference lists 5 mL for a 500 mg vial.

Vial2 mL water5 mL water10 mL water
100 mg vial50,000 mcg/mL500 mcg per unit20,000 mcg/mL200 mcg per unit10,000 mcg/mL100 mcg per unit
500 mg vial250,000 mcg/mL2.5 mg per unit100,000 mcg/mL1 mg per unit50,000 mcg/mL500 mcg per unit
1,000 mg vial500,000 mcg/mL5 mg per unit200,000 mcg/mL2 mg per unit100,000 mcg/mL1 mg per unit

Before you reconstitute

Check the vial label for the actual amount of peptide, because the calculation depends on it. Use an insulin syringe marked U-100 so that 100 units equal 1 mL, and write down how much water you added so the concentration is known later.

For what NAD+ is, how it is thought to work and what the research shows, see the NAD+ reference page. For how its evidence compares with other peptides, see the peptide tier list.

Frequently asked questions

How much BAC water goes in a 500 mg NAD+ vial?

The reconstitution reference in the PeptidesAI app lists 5 mL for a 500 mg vial, which makes 100,000 mcg/mL. The water volume is a choice, not a fixed rule: more water makes a weaker solution and larger draws, and the total amount of peptide in the vial stays the same.

How much is one unit of NAD+ on an insulin syringe?

It depends on the concentration. A 500 mg vial with 5 mL of water is 100,000 mcg/mL, so one unit on a U-100 syringe (0.01 mL) holds 1 mg. The table on this page shows other vial and water combinations.

What vial sizes does NAD+ come in?

The PeptidesAI app lists 100 mg, 500 mg, 1,000 mg vials for NAD+. Use the calculator's vial field for any other size.

Does this calculator tell me how much to use?

No. It converts an amount you already have into syringe units and millilitres. It does not suggest an amount, and nothing on this page is medical advice.

Save this vial in the app

PeptidesAI remembers your vial and water, shows the units for every dose you log, and reminds you when it's time. Free to download on iPhone.

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